How to quantify factors degrading DNA in the environment …

Using 10 environmentally diverse bat roosts as a case study, we developed a robust modeling pipeline to quantify the environmental factors degrading eoDNA, predict …

Cloud-Point Extraction Combined with Thermal Degradation …

Cloud-Point Extraction Combined with Thermal Degradation for Nanoplastic Analysis Using Pyrolysis Gas Chromatography-Mass Spectrometry ... of nanoplastics remains an unsolved challenge. Herein, for the first time, a Triton X-45 (TX-45)-based cloud-point extraction (CPE) was proposed to preconcentrate trace nanoplastics in …

(PDF) Exploitation minière artisanale et semi-mécanisée de …

Le coût du gramme d'or est fixé selon les mécanismes de l'ANEEMAS, en tenant compte des impuretés aurifères, au niveau national, et, en tenant compte des fluctuations internationales du coût de l'or. Certain·e·s propriétaires de galerie et collecteur·euse·s d'or estiment que le coût du gramme se situe entre 17 500 et 25 000 ...

A scientific approach to extraction methods and stability …

The compounds were stable in the pH range of 3.2–7, with the most considerable degradation observed at pH 2.4, followed by pH 8. ... According to Li et al. (2016), the extraction of pigments by MAE from Black currant (Ribes nigrum) showed good yields, and the optimum conditions of the process were 551 W and an exposure time of …

Extraction of Flavonoids From Natural Sources Using …

However, these techniques are characterized by the use of large amounts of organic solvents with high purity, lower extraction yields, low selectivity, long extraction times, thermal degradation of target compounds, associated environmental concerns and costs, compared to other techniques ( et al., 2010; Galanakis, 2012; Farzaneth and …

Targeted protein degradation via intramolecular bivalent glues

Targeted protein degradation is a pharmacological modality that is based on the induced proximity of an E3 ubiquitin ligase and a target protein to promote target ubiquitination and proteasomal ...

Effect of temperature in the degradation of cannabinoids: …

The kinetic degradation of CBD was evaluated in this way, observing a degradation of 0.22 μg/L per second. At the same time, the kinetics of the appearance of Δ 9-THC demonstrates the intermediate nature of this cannabinoid, being degraded at 0.03 s −1 μM −1. The degradation of CBD also produced CBN and CBE as by-products.

Techniques for extraction and isolation of natural …

The high temperature and long extraction time in the Soxhlet extraction will increase the possibilities of thermal degradation. Wei et al. obtained ursolic acid (52, Fig. 6) from the TCM Cynomorium (Cynomorii Herba) with a yield of 38.21 mg/g by Soxhlet extraction . The degradation of catechins in tea was also observed in Soxhlet …

Biomass degradation: Challenges and strategies in extraction …

Moreover, this treatment generated the high purity of extraction hemicellulose (95.60%) and degradation of β-glycosidic bonds could be prevented indeed. Approximately, 40.6% of lignin and 54.7% of hemicellulose can be extracted over p-TsOH method as investigated by Wei et al [29] to lower the lignin content.

Extraction of highly degraded DNA from ancient bones, …

Here, we provide a highly versatile silica-based DNA extraction protocol that enables the retrieval of short (≥35 bp) or even ultrashort (≥25 bp) DNA fragments from …

Development course of separating rare earths with acid …

Extraction and interfacial kinetics of Nd 3+ and Sm 3+ with HEH/EHP_kerosene in a hollow fiber membrane extractor were studied by Zhang et al. 140, 141 The results show that the extraction reactions in the hollow fiber membrane extractor are the same as those in the liquid–liquid extraction, which can be expressed as a quasi …

Green synthesis of iron oxide nanoparticle using Carica …

The synthesis scheme of iron oxide NPs is shown in Figure 1 a. The various phytochemicals like polyphenols, flavonoids, glycosides, and tannins present in the leaf extract equally act as reducing and stabilizing agents for the synthesis of NPs (Juárez-Rojop et al., 2014).The formation of black color precipitates occurred due to the …

Determination of pesticides and their degradation products …

The degradation products formed can constitute an extremely complex blend of substances (e.g., Lerch et al. [25] reported that 28 different metabolites of trifluralin were formed in soils, and, because standards were available for only trifluralin and six of its metabolites, the other TPs can only be identified from MS/MS data, but not ...

A review of methods for extraction, removal, and stimulated degradation …

The state-of-the-art methods for the extraction, separation, identification and quantification of nanoplastic in the environment have been recently reviewed by Li et al. [173]. The degradation pathways and products depend on the polymer type, ultimately leading to low molecular weight polymer fragments [ 27 ].

Eggplant Peels as a Valuable Source of Anthocyanins: Extraction …

Qiu et al. noticed that k values increased with the temperature in the 50–80 °C range after the thermal treatment of purple potato extract. Similar to our study, Oancea et al. noticed that the k values for the antioxidant activity of elderberry extract were lower when compared with the TAC thermal degradation, but also increasing with the ...

DNA damage in preserved specimens and tissue samples: a …

Previous studies have described techniques for improved DNA extraction from such samples or focused on the effect of damaging agents – such as light, oxygen …

DNA Degradation and Its Defects

DNase II and Its Degradation of the DNA of Apoptotic Cells. The enzyme that digests DNA in macrophage lysosomes is deoxyribonuclease II (DNase II), also called DNase IIα or …

A novel mechanism for the loss of mRNA activity in lipid

Lipid nanoparticle (LNP)-formulated mRNA vaccines were rapidly developed and deployed in response to the SARS-CoV-2 pandemic. Due to the labile nature of mRNA, identifying impurities that could ...

Extraction and application of keratin from natural resources: …

Feroz et al. carried out extraction by using feathers at a temperature of 160–200 °C for 20 min and received a yield of 71.83%. Steam explosion process ... Degradation of keratin and its disposal can cause a harmful impact on the environment. Therefore, extensive investigations focusing on keratin-waste utilisation have been …

The drivers and impacts of Amazon forest …

Underlying drivers (a few of which are shown in gray at the bottom) stimulate disturbances (timber extraction, fire, edge effects, and extreme drought) that cause forest degradation. A satellite illustrates the …

Micro- and Nanoplastics in Environment: Degradation

Polymer degradation can be quantified by measuring changes in mass, which is the simplest and most straightforward method. Degradation in soil (Wu et al. 2021; Melo-Agustín et al. 2022), compost (Liu et al. 2021; Pashaei et al. 2022), and labs with enriched microorganisms has all been assessed by measuring mass loss (Pignattelli et …

RNA-seq: impact of RNA degradation on transcript …

Degradation of RNA transcripts by the cellular machinery is a complex and highly regulated process. In live cells and tissues, the abundance of mRNA is tightly regulated, and transcripts are degraded at different rates by various mechanisms [], partially in relation to their biological function [2–5].In contrast, the fates of RNA transcripts in …

Comparison of three different DNA extraction methods from …

Decay is directly associated with the decomposition of soft tissues and also the degradation of genetic material inside cells. 1. Decomposition rates vary depending …

Analytical tools for the analysis of β-carotene and its degradation …

Recently, Nakornriab et al. described supercritical fluid extraction (SFE) with CO 2 at 6,000 psi and 45°C to 60°C, off-line hyphenated to HPLC-ESI-MS as an innovative extraction method for β-carotene. The SFE method allowed for recoveries between 92% and 101%, while enabling rapid extraction time and avoiding solvent contamination.

Silica nanoparticle based techniques for extraction, detection…

Pesticides are generally detected by employing high-end expensive techniques such as Gas Chromatography (GC) and High Performance Liquid Chromatography (HPLC) [2].Their degradation is mediated by plants and microorganisms through their metabolites [3].In addition to the abovementioned conventional methods …

An overview of DNA degradation and its implications in …

DNA degradation, despite its challenges, is a potent tool in forensic science. Understanding the factors and mechanisms influencing DNA degradation is essential for its effective utilization in criminal casework.

Sustainable Biodegradation and Extraction of Keratin with …

Green Phenomenon to Extract Keratin Proteins with Liquid Deep-Eutectic Solvent (Urea-Sodium Acetate) A huge amount of feathers in form of waste are generated in the poultry industries which are re-cycled, incinerated, or dumped in landfills (Nuutinen et al. 2019).However, the green extraction of protein, i.e., keratin from the feathers provides a …

Extraction de l'or : nouvelles solutions et méthodes plus écologiques

1) Remplacement du cyanure par la fécule de maïs. Ce dérivé d'amidon extrait l'or du minerai, de façon plus écologique. On remplace le cyanure en utilisant l'amidon issu du maïs pour extraire le métal lourd. 2) Utilise cette technique pour dissocier le métal précieux des déchets électroniques.

Processing and extraction methods of medicinal cannabis: a …

Namdar et al. reported that for cannabis plant extraction, the ratio and the nature of the solvents can determine the evaporation time after extraction, which should be minimized. A mixture of polar and non-polar solvents achieved the highest yield for all the compounds in the cannabis plant (Namdar et al. 2018 ).

Natural Resource Extraction, Armed Violence, and Environmental Degradation

The social disorder that often resulted from structural adjustment contributed in some cases to higher violent crime rates and, more rarely, to armed rebellion (Bello et al., 1999). However, poverty, hunger, disease, and social disorder are also forms of violence that can have devastating physical and psychological effects.

L'extraction du pétrole : tout ce qu'il faut savoir

L' extraction de pétrole débute par une étude des sols pour être capable d'identifier les gisements pétroliers exploitables. D'abord, seul un contexte particulier va permettre de s'intéresser à un territoire. Il faut que ce soit un lieu avec un climat chaud, à proximité d'un fleuve et suffisamment loin des montagnes.